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. 2024 Jul 2;46(5):4017–4035. doi: 10.1007/s11357-024-01233-w

Table 1.

Effects of shifting Canton-S female flies from a high (H)- to a low (L)-calorie diet on longevity compared to longevity of flies on lifelong high-calorie diet

Food Time on L diet N
(n censored) 
Mean LS
(% change)
X2 p Maximal LS (% change)
H Lifelong 402 (12) 46.7 (− 91.2) 751.9572  < 0.0001* 68.2 (− 62.2)
L Lifelong 439 (6) 89.3 110.6
H Lifelong 402 (12) 46.7 (− 84.2) 423.5131  < 0.0001* 68.2 (− 53.9)
HLD20 D20 207 (4) 86.0 105
H Lifelong 402 (12) 46.7 (− 26) 47.8706  < 0.0001* 68.2 (− 59)
HLD50 D50 217 (7) 59.1 108.2
H Lifelong 402 (12) 46.7 (− 13.9) 16.5431 0.0742 68.2 (− 53.8)
HLD60 D60 211 (10) 53.2 104.9

L low-calorie diet, H high-calorie diet, HLD20 flies shifted from a high- to a low-calorie diet at day 20, HLD50 flies shifted from a high- to a low-calorie diet at day 50, HLD60 flies shifted from a high to low-calorie diet at day 60

N = Number of flies in experiment used for calculating mean and maximal lifespan

n censored = number of flies that died between 0 and 10 days, and are not included in listed N and are not included in calculation of mean and maximal lifespan. The total number of flies in experiments on day 0 is N plus n flies)

*Statistically significant