Table 2.

Cq values of exogenous and endogenous miRNAs in plasma samples retrieved from the IRCCS Synlab SDN biobank dating from 2018–2023 were compared to a positive control (fresh plasma without any freezing steps) and a negative control (a sample degraded and kept at 4 ℃ for one month).

miRNAs	Sample 1–12 mean ± ds	Positive control mean ± ds	Negative control mean ± ds
UniSp2	19.24 ± 0.55	19.5 ± 0.67	19.79 ± 0.06
UniSP6	19.63 ± 0.08	19.65 ± 0.23	19.60 ± 0.11
hsa-miR-16-5p	22.63 ± 1.78	24.43 ± 0.31	30.20 ± 0.95
hsa-miR-21-5p	24.49 ± 1.31	23.49 ± 0.45	33.27 ± 0.88
hsa-miR-150-5p	28.71 ± 1.39	28.54 ± 1.28	33.85 ± 0.23