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PDE9 but not PDE5 expression is significantly upregulated in aged animals (n = 6), when compared to young controls (n = 5). A Representative images of Western immunoblot for detecting protein expression of PDE5, PDE9 and the picture of total unstained gel for the total protein loading control. B, C Quantified protein expression for PDE9 and PDE5 respectively, normalized for a total protein. Data presented as means ± SEM and analyzed using unpaired t-test; * p < 0.05
