Fig. 2.
Survival and functional healthspan. A Kaplan-Meier survival plots and B violin distribution plots by group for (22(PF27) [Ta 22°C, pair-fed to 27°C] or AL27 [Ta 27°C, ad libitum fed]) in male C57BL/6J mice (n=60/group). Cage side battery of functional and physical assessments including C ledge test, D kyphosis, E gait analysis, and F hind-limb clasping by age at assessment (weeks 56, 64, 76, 88, 100, 113, 130, 142, 154). Scores (mean ±SD) plotted by group for (22(PF27) [Ta 22°C, pair-fed to 27°C] (starting n=56, decreasing with mortality) or AL27 [Ta 27°C, ad libitum fed]) (n=58) in male C57BL/6J mice. Higher numeric scores reflect greater impairment (C–F)