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. 2024 May 22;33(17):1524–1539. doi: 10.1093/hmg/ddae087

Figure 1.

Figure 1

Single nuclei RNA-seq of the OVT73 sheep striatum. (A) Experimental design. Nuclei were extracted from the striatum of 12 sheep (6 OVT73 and 6 controls) and pooled to form 7 nuclei suspensions. Single nuclei RNA libraries were generated from the sample multiplexed suspensions and sequenced. Reads were demultiplexed based on the natural genetic variation between pooled samples. Panel image created with BioRender.com. (B) UMAP visualisation of clusters identified and annotated by cell marker gene expression. (C) Proportions of identified cell types across the 12 animals. A significant decrease in oligodendrocytes between OVT73 and control cases was observed (***ANOVA, P < 0.001) (D) dot plot of selected cell-type enriched markers for annotated cell types in the sheep striatum.