Fig. 2.

BCKDK promotes GBM proliferation ex vivo and in vivo. A, the protein levels of BCKDK in 6 glioma cell lines were analyzed by WB. B, the effect of BCKDK knockdown on the expression of its downstream signaling molecules was examined by WB. The data are representative pictures of three replicates. C, Growth curve of BCKDK knockdown cell lines were determined by MTT assay. The data are presented as the mean ± SD of three replications. D, Soft agar of BCKDK knockdown cell lines. Scale bar: 200 μm. The data are presented as the mean ± SD of three replications. E, Tumor size by in vivo bioluminescence in xenograft tumor-bearing BALB/c-nu mice with silenced BCKDK cell lines. F, The intracranial tumor sections of control group and experimental group of nude mice were stained with HE. G, the OS curves of BALB/c-nu mice with GBM xenografts are built according to the Kaplan-Meier methods. n = 8 (***p < 0.001). H, the effect of stable overexpression of BCKDK on the expression of its downstream signaling molecules was examined by WB. The data are representative pictures of three replicates. I, Colony formation assay of control and BCKDK stable cells. J, Tumor size of BCKDK stable overexpression cell lines in xenograft mice was detected using bioluminescence on day 14 and day 21. K, the OS curves of C57 mice with GBM xenografts are built according to the Kaplan-Meier methods. n = 8 (*p < 0.05).