Table 2.
Box–Behnken experiments design matrix with experimental and value of 1,3-propanediol yield for culture medium component.
| Run | X1 | X2 | X3 | X4 | Crude Glycerol (g/L) |
(NH4)2SO4 (g/L) |
MgSO4.7H2O (g/L) | CaCl22H2O (g/L) | 1,3-PDO (g/L) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | −1 | −1 | 0 | 0 | 25 | 2.5 | 0.5 | 0.05 | 15.00 |
| 2 | 1 | −1 | 0 | 0 | 75 | 2.5 | 0.5 | 0.05 | 24.98 |
| 3 | 0 | 0 | −1 | 1 | 50 | 5 | 0.25 | 0.075 | 25.91 |
| 4 | −1 | 1 | 0 | 0 | 25 | 7.5 | 0.5 | 0.05 | 20.24 |
| 5 | 0 | 0 | 1 | −1 | 50 | 5 | 0.75 | 0.025 | 23.84 |
| 6 | 1 | 1 | 0 | 0 | 75 | 7.5 | 0.5 | 0.05 | 38.54 |
| 7 | 0 | 0 | 1 | 1 | 50 | 5 | 0.75 | 0.075 | 27.67 |
| 8 | −1 | 0 | 0 | −1 | 25 | 5 | 0.5 | 0.025 | 18.84 |
| 9 | −1 | 0 | 0 | 1 | 25 | 5 | 0.5 | 0.075 | 20.32 |
| 10 | 0 | −1 | 0 | 1 | 50 | 2.5 | 0.5 | 0.075 | 28.24 |
| 11 | 1 | 0 | 0 | −1 | 75 | 5 | 0.5 | 0.025 | 31.24 |
| 12 | 1 | 0 | 0 | 1 | 75 | 5 | 0.5 | 0.075 | 34.24 |
| 13 | 0 | −1 | −1 | 0 | 50 | 2.5 | 0.25 | 0.05 | 24.00 |
| 14 | −1 | 0 | −1 | 0 | 25 | 5 | 0.25 | 0.05 | 20.84 |
| 15 | 0 | 0 | −1 | −1 | 50 | 5 | 0.25 | 0.025 | 19.24 |
| 16 | 0 | −1 | 1 | 0 | 50 | 2.5 | 0.75 | 0.05 | 17.00 |
| 17 | 0 | 0 | 0 | 0 | 50 | 5 | 0.5 | 0.05 | 25.30 |
| 18 | 0 | 0 | 0 | 0 | 50 | 5 | 0.5 | 0.05 | 26.24 |
| 19 | 0 | 0 | 0 | 0 | 50 | 5 | 0.5 | 0.05 | 25.14 |
| 20 | 0 | 0 | 0 | 0 | 50 | 5 | 0.5 | 0.05 | 24.74 |
| 21 | 0 | 0 | 0 | 0 | 50 | 5 | 0.5 | 0.05 | 24.27 |
| 22 | 0 | 1 | −1 | 0 | 50 | 7.5 | 0.25 | 0.05 | 29.24 |
| 23 | 0 | 1 | 1 | 0 | 50 | 7.5 | 0.75 | 0.05 | 29.94 |
| 24 | −1 | 0 | 1 | 0 | 25 | 5 | 0.75 | 0.05 | 15.84 |
| 25 | 1 | 0 | −1 | 0 | 75 | 5 | 0.25 | 0.05 | 32.94 |
| 26 | 1 | 0 | 1 | 0 | 75 | 5 | 0.75 | 0.05 | 35.00 |
| 27 | 0 | −1 | 0 | −1 | 50 | 2.5 | 0.5 | 0.025 | 14.24 |
| 28 | 0 | 1 | 0 | −1 | 50 | 7.5 | 0.5 | 0.025 | 27.24 |
| 29 | 0 | 1 | 0 | 1 | 50 | 7.5 | 0.5 | 0.075 | 23.00 |