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pSI1 plasmid extracted from Streptococcus iniae QMA0139, visualized 0.5% TAE agarose gel. Lane 1 – Quick‐Load 1 kb Extend DNA Ladder (NEB); Lane 2 – 1 μg of undigested pSI1; Lane 3 ‐ 1 μg of pSI1 digested with NsiI (NEB); Lane 3 ‐ 1 μg of pSI1 digested with AfIII (NEB); Lane 4 – Fast DNA Ladder (NEB).
