Table 1.
Combinations of methods for cell lysis and DNA recovery used for DNA extraction (References are provided for combinations applied in the published protocols).
| Method# | Cell lysisa | DNA recovery | Hazard/toxicity |
|---|---|---|---|
| 1. | SDS/proteinase K/CTAB | Phenol‐chloroform | Toxic lysis (CTAB) and toxic recovery (phenol, chloroform) (Wilson, 2001) |
| OMO/proteinase K | Nontoxic lysis and toxic recovery (phenol, chloroform) | ||
| 2. | OMO | Ethanol | Nontoxic lysis and recovery (Mirnejad et al., 2012) |
| SDS/proteinase K | Nontoxic lysis and recovery | ||
| 3. | SDS/proteinase K | Cellulose dipstick | Nontoxic lysis and recovery (Mason & Botella, 2020) |
| OMO/proteinase K | Nontoxic lysis and recovery | ||
| 4. | GITC | Isopropanol/magnetic beads | Toxic lysis (GITC) and nontoxic recovery (Oberacker, Stepper, Bond, Höhn, et al., 2019) |
| SDS/proteinase K | Nontoxic lysis and nontoxic recovery | ||
| OMO/proteinase K | Nontoxic lysis and nontoxic recovery | ||
| 5. | SDS | PEG8000/magnetic beads | Nontoxic lysis and recovery (Mayjonade et al., 2016) |
| OMO | Nontoxic lysis and recovery |
Abbreviations: CTAB, cetyltrimethylammonium bromide; GITC, guanidinium isothiocyanate; OMO, OMO detergent laundry powder; PEG8000, polyethylene glycol 8000; SDS, sodium dodecyl sulfate.
a
In all cases performed with and without lysozyme pretreatment.