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. 2024 Jul 24;13(7):1291–1304. doi: 10.21037/gs-24-173

Table 2. Studies of healthy and diseased populations denote relevant immunological mechanisms associated with wet cupping.

Category Author Year Cohorts Immunomodulation
Normal Dons'koi (42) 2016 Health population Decreased NK cell activity
No change in CD4+ T cells and CD8+ T cells
Obohat (44) 2020 Health population Decreased fasting blood glucose and uric acid
No change in WBC, ESR, or ferritin
Soleimani (52) 2020 Health population Increased expression of transcription factors: GATA-3, RORγt, FOXP3
Decreased T-bet/GATA-3 (Th1/Th2)
Disease Ahmed (40) 2005 Rheumatoid arthritis Increased NK cells and WBC
Decreased RF, ESR, CRP, and sIL-2R
Decreased CRP (hs-CRP)
Abdulaziz (41) 2021 Female chronic pelvic pain Decreased CRP (hs-CRP)
Obeid (43) 2022 Hashimoto’s thyroiditis Decreased anti-TPO and anti-Tg
Decreased ESR, prolactin, and TSH. No change in T4
El-Shanshory (53) 2022 Thalassemia Increased CD4/CD8 ratio and TAC/MDA ratio
Yin (54) 2022 Functional diarrhea Increased CD4+ T cells, CD8+ T cells, and T17 helper lymphocytes
Zhang (55) 2006 Bronchial asthma Increased C3, C4, IgG, IgM, IgA, CD4+ T cells, CD4+/CD8+ ratio, IL-2, and IFN-γ
Decreased IgE, IL-4, IL-10, and CD8+ T cells
Sun (56) 2016 Acute scapulohumeral peri-arthritis Decreased 5-HT and PGE2
El-Domyati (57) 2013 Dermatologic diseases Increased C3
Decreased IgE and IL-2
Tian (58) 2013 Postherpetic neuralgia Decreased P substance

WBC, white blood cell; ESR, erythrocyte sedimentation rate; NK, natural killer; RF, rheumatoid factor; CRP, C-reactive protein; sIL-2R, soluble interleukin-2 receptor; hs-CRP, high-sensitivity CRP; TPO, thyroid peroxidase; Tg, thyroglobulin; TSH, thyroid-stimulating hormone; TAC/MDA, total antioxidant capacity/malondialdehyde; IFN, interferon; 5-HT, 5-hydroxy-tryptamine; PGE2, prostaglandin E2.