Figure 1.

Postnatal deletion of NCOR1 and SMRT in intestinal epithelial cells leads to sustained weight loss, hypoglycemia, and increased mortality. A) Shown are the genotypes that were studied, control and NCOR1/SMRT I-DKO mice. B) Experimental timeline. Tamoxifen was administered to male mice at age 8 to 9 weeks for 5 days and mice then underwent glucose tolerance test followed by metabolic phenotyping and EchoMRI. C) Percentage of male control and I-DKO mice that survived to 20 days (n = 7 control mice, 7 [out of 18] I-DKO mice survived). D) Body weight; E) blood glucose with area under the curve analysis; and F) body temperature from the surviving mice (n = 7 control, 7 I-DKO). G) Hematoxylin and eosin staining from representative isolated jejunum. H) Villi length (n = 5 per genotype). I) Intestine length (n = 7 control, 5 I-DKO). J) Quantitative polymerase chain reaction for Ncor1 and Smrt was performed on RNA extracted from intestinal epithelial cells (IECs) (n = 6 per genotype). Data are shown as mean ± SEM, 2-way analysis of variance for time (#P < .0001) and genotype ($P < .0001) for survival, body weight, glucose, and temperature analysis, otherwise unpaired t test (****P < .0001).