Fig. 2. Genes critical for drug resistance to NL101 identified by clustered regularly interspaced short palindromic repeats (CRISPR) library screen. (a) Landscape of a CRISPR library screen. (b) Heatmap of genes identified by genome-wide CRISPR library screen. (c, d) Western blot analyses of mammalian target of rapamycin (mTOR) signaling pathway in cell lines exposed to NL101 for 24 h. sgRNA: single-guide RNA; PCR: polymerase chain reaction; PTEN: phosphatase and tensin homologous; p: phosphorylated; AKT: protein kinase B; S6: ribosomal S6 protein; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.
