A: divanillyltetrahydrofuran ferulate; B: trans-ferulic acid; C: tryptamine; D: neuraminic acid. Each plot represents the ROC curve and metabolic box plot of the same metabolite in the Control vs POI group (above) and ZS + POI vs POI group (below). A1, B1, C1, D1: ROC curve plot in the Control vs POI group; A2, B2, C2, D2: box plot in the Control vs POI group; A3, B3, C3, D3: ROC curve plot in the ZS+POI vs POI group; A4, B4, C4, D4: box plot in the ZS + POI vs POI group. In the ROC curve plot, the area surrounded by the blue dot solid line and the straight line (x = 1, y = 0) is AUC, the closer the AUC value is to 1, the higher the accuracy of the prediction. In the box plot, the content distribution of metabolites in each group was shown. Control: normal feeding, without any treatment; POI: POI model was induced by intraperitoneal injection of CTX (2 mg/mL) for 15 d; SA + POI: sham acupuncture intervention in CTX-induced POI rats for 4 weeks; ZS + POI: Zhibian (BL54) through Shuidao (ST28) acupuncture intervention in CTX-induced POI rats for 4 weeks. ROC: receiver operating characteristic curve; POI: premature ovarian insufficiency; ZS: Zhibian (BL54) through Shuidao (ST28) acupuncture; AUC: area under roc; CTX: cyclophosphamide.