Table 4.
Effect of "Zhibian (BL54) through Shuidao (ST28)" acupuncture on potential biomarkers in the serum of rats with POI
| Name | Formula | Mass-to-charge ratio (m/z) |
RT (min) |
Control vs POI | POI vs ZS+POI | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| FC |
P
value |
VIP | Trend | FC | P value | VIP | Trend | |||||
| Divanillyltetrahydrofuran ferulate | C30H32O8 | 1099.4293 | 11.4854 | 0.2409 | 0.0001 | 2.1464 | ↓a | 4.4102 | 0.0000 | 2.2110 | ↑c | |
| Rolitetracycline | C27H33N3O8 | 1099.4546 | 13.5905 | 0.1087 | 0.0011 | 2.0491 | ↓b | 6.5169 | 0.0000 | 2.1883 | ↑c | |
| Trans-ferulic acid | C10H10O4 | 239.0564 | 2.9167 | 0.6022 | 0.0006 | 1.9304 | ↓a | 1.5964 | 0.0027 | 1.8209 | ↑b | |
| Tryptamine | C10H12N2 | 159.0912 | 2.4098 | 0.7189 | 0.0023 | 1.8795 | ↓b | 4.2828 | 0.0000 | 2.2682 | ↑c | |
| Neuraminic acid | C9H17NO8 | 288.0653 | 2.0602 | 0.4761 | 0.0038 | 1.8148 | ↓b | 1.6599 | 0.0095 | 1.6740 | ↑b | |
| Eupatilin | C18H16O7 | 343.0840 | 4.2009 | 0.5563 | 0.0030 | 1.7867 | ↓b | 2.0420 | 0.0015 | 1.8760 | ↑b | |
Notes: control: normal feeding, without any treatment; POI: POI model was induced by intraperitoneal injection of CTX (2 mg/mL) for 15 d; ZS + POI: Zhibian (BL54) through Shuidao (ST28) acupuncture intervention in CTX-induced POI rats for 4 weeks. POI: premature ovarian insufficiency; CTX: cyclophosphamide; ZS: Zhibian (BL54) through Shuidao (ST28) acupuncture; FC: fold change; VIP: variable importance in projection; RT: retention time. The difference in metabolites between the control and experimental groups was more than 1 time, and the difference was considered significant. FC ≥ 1 (when the FC threshold is 1, a screening FC ≥ 1 is upregulated and a screening FC ≤ 1 is downregulated). The VIP value represents the influence intensity of the difference between the corresponding metabolites in the classification and discrimination of each group of samples in the model. It is generally believed that the metabolites with VIP ≥ 1 are significantly different. P value is the P value of t test, metabolites with P value < 0.05 are generally considered to be significantly different, in this study, P value < 0.01 of t test was used to screen differential metabolites, aP < 0.001, bP < 0.01, cP < 0.0001.