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. 2024 Jun 17;44(4):703–712. doi: 10.19852/j.cnki.jtcm.20240617.002

Figure 2. Effect of XLHZ on proliferation and migration of MNNG-induced GES-1 cells.

Figure 2

A: EdU assay was used to detect cell proliferation (magnification × 200). The proliferating cells were fluorescently stained with EdU (Green). The nuclei were stained with DAPI (Blue). the higher the cell proliferation rate, the brighter the green in the plot. A1: EdU of the control group; A2: DAPI of the control group; A3: merge of the control group; A4: EdU of the model group; A5: DAPI of the model group; A6: merge of the model group; A7: EdU of the XLHZ group; A8: DAPI of the XLHZ group; A9: merge of the XLHZ group. B: Quantitative analysis of proliferating cells. C: Wound healing assay was used to detect cell migration in the normal, model and XLHZ groups (magnification × 200). C1-C5: The migration of cells in the normal group was observed at 0, 12, 24, 48, 72 h. C6-C10: The migration of cells in the model group was observed at 0, 12, 24, 48, 72 h. C11-C15: The migration of cells in the XLHZ group was observed at 0, 12, 24, 48, 72 h. D: quantitative analysis of cells migration rate at 0, 12, 24, 48, 72 h. normal group: drink and eat freely + the same volume of normal saline was given by gavage; model group: MNNG, 180 μg/mL + 2% sodium salicylate, 1 mL/100 g per day + hunger and satiety disorders; XLHZ: model group + XLHZ, 36 g·kg-1·d-1. XLHZ: Xianglian Huazhuo formula; MNNG: N-Methyl-N'-Nitro-N-Nitrosoguanidine; GES-1: human gastric mucosal epithelial cells; EdU: 5-Ethynyl-2’- deoxyuridine; DAPI: 4’,6-Diamidino-2’-phenylindole. Statistical analyses were measured using one-way analysis of variance followed by post hoc Bonferroni correction for multiple comparisons. Data were presented as mean ± standard deviation (n = 3). Compared with the normal group, aP < 0.05, bP < 0.01; Compared with the model group, cP < 0.05; dP < 0.01.