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. 2004 Oct 26;383(Pt 3):483–490. doi: 10.1042/BJ20040519

Figure 2. JNK changes the substrate specificity of GST spliceforms.

Figure 2

GST and JNK were incubated in 1:1 molar ratio for 5 min at room temperature. GST activity was measured using various hydrophobic co-substrates. The percentage change in GST activity (y-axis) was determined by comparing the reactions in the presence and absence of JNK. The specific activity of each GST for the substrates in the absence of JNK is shown in Table 3. There was no activity change in GSTD1-1 activity using CDNB as the co-substrate due to the lack of a JNK effect (Table 1). Results shown are representative of at least four independent experiments with similar results.