Figure 2. Detection of mitochondrial ND activity in mitochondria from 143B, rho°, C4T and C9T cells by BN-PAGE.
The gel was stained for NADH–NBT oxidoreductase activity as described in the Experimental section. Identical amounts of mitochondrial proteins (200 μg) were loaded. Lane 1, 143B cells; lane 2, rho° cells; lane 3, C4T cells; lane 4, C9T cells. The molecular masses of the proteins, indicated on the left, were calculated by comparison with known standards (urease and apoferritin from Sigma).