Skip to main content
. 2004 Oct 26;383(Pt 3):501–506. doi: 10.1042/BJ20040864

Figure 1. Hydrolysis of BK by cathepsin K.

Figure 1

Human cathepsin K was incubated with BK (enzyme/substrate molar ratio 1:2500) at 37 °C, in 0.1 M phosphate buffer, pH 6.0, containing 2 mM DTT and 1 mM EDTA for periods of 0–5 min (black bar). The same procedure was repeated in 0.1 M phosphate buffer, pH 7.4, containing 2 mM DTT and 1 mM EDTA (white bar). Hydrolysis products were further fractionated by reverse-phase HPLC (C18 Brownlee ODS-032 column) (see the Experimental section for details). For the sake of clarity, the residual amount of BK was normalized.