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. 2004 Oct 26;383(Pt 3):517–527. doi: 10.1042/BJ20040721

Figure 1. Expression of His6–BjHMGS1 in E. coli BL21 Star (DE3) pLysS cells.

Figure 1

Coomassie Blue-stained gel of soluble protein from E. coli bearing pBj75 before induction with 0.5 mM IPTG (lane 1) and 4 h post-induction (lane 2). Western-blot analysis using anti-peptide antibodies against His6–BjHMGS1 using similar cultures before (lane 3) and after (lane 4) IPTG-induction. SDS/PAGE of Ni2+-NTA purified His6–BjHMGS1 stained with Commassie Blue (lane 5). The arrowheads denote the 53 kDa His6–BjHMGS1. M, molecular-mass markers.