Table 1. Synthetic oligonucleotide primers used for in vitro site-directed mutagenesis.

Codons for the changed amino acids are underlined. Nucleotides represented in italics indicate the product point mutations.

Primer name	Primer sequence
Y35A	5′-GCAAAGGGAAAGCCACCATTGGAC-3′
R77A	5′-CAAGCAAATTGGGGCTCTTGAAGTAGGC-3′
K88A	5′-GACTGTCATTGACGCGAGCAAGTCCATC-3′
S89A	5′-CTGTCATTGACAAGGCCAAGTCCATCAAAAC-3′
K90A	5′-CATTGACAAGAGCGCGTCCATCAAAACC-3′
N115A	5′-CCAATGCTTGTGCTGGTGGAACTGC-3′
C117A	5′-CGACCAATGCTGCTTATGGTGGAACTG-3′
Y118A	5′-CCAATGCTTGTGCTGGTGGAACTGC-3′
R157A	5′-GAAGGGCCCGCAGCGCCCACTGGAGG-3′
H188N	5′-GAAGCCACATGGCTAATGTCTATGACTTTTAC-3′
C212S	5′-CTTTCACAGACATCCTACCTTATGGC-3′
S356A	5′-GTGGTTATGTTCGCATATGGAAGTGG-3′
S359A	5′-GTTCTCATATGGAGCTGGTTCAACCGC-3′