. 2004 Oct 26;383(Pt 3):517–527. doi: 10.1042/BJ20040721

Table 2. Purification of His₆–BjHMGS1 from a 1 litre culture of pBj75-transformed E. coli BL21 Star (DE3) pLysS cells following induction with 0.5 mM IPTG.

	Crude cell extract	Ni²⁺-NTA purified fraction
Volume (ml)	24.5	5.5
Total protein (mg)	43.5	4.95
Total activity (μmol·min⁻¹)	1.13	0.52
Specific activity (μmol·min⁻¹·mg⁻¹)	0.02	0.10
Yield (%)	100.00	46.01

Enzyme activity was measured in the presence of equimolar concentrations of [¹⁴C]acetyl-CoA and AcAc-CoA at 30 °C.

Table 2. Purification of His6–BjHMGS1 from a 1 litre culture of pBj75-transformed E. coli BL21 Star (DE3) pLysS cells following induction with 0.5 mM IPTG.