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. 2004 Oct 26;383(Pt 3):581–588. doi: 10.1042/BJ20040654

Figure 4. Roles of PLCγ2, Syk and SLP-76 in collagen-induced signalling in WT and γ−/− platelets.

Figure 4

Tyrosine phosphorylation of (A) PLCγ2, (B) Syk and (C) SLP-76 in WT and γ−/− platelets is shown. The proteins were immunoprecipitated (IP) from lysed aliquots of platelets following stimulation for 2.5 min with collagen (50 μg/ml), CRP (5 μg/ml), U46619 (1 μM), or a combination of collagen and U46619 in the absence or presence of Ha1/29 (20 μg/ml). Lotrafiban (10 μM) was present in all cases. Densitometric data are shown for PLCγ2 (n=4), and expressed as means±S.E.M. of relative integrated absorbance (IOD) units. No densitometric data are shown for Syk or SLP-76, since no consistent measurable phosphorylation above background was ever observed in the γ−/− platelets. Representative Western blots (WB) are shown for PLCγ2, Syk (n=3) and SLP-76 (n=2).