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. 2004 Jun 22;381(Pt 1):35–42. doi: 10.1042/BJ20031933

Figure 1. Separation of the enzymes acting on D- and L-2-hydroxyglutarate by chromatography on DEAE-Sepharose.

Figure 1

Activity (•) was measured with radiolabelled DL-2-hydroxy[2-3H]glutarate on 25 μl of each fraction. The absorbance A280 was also measured (▵). The oblique line indicates the NaCl gradient.