Figure 8. Decreased mRNA levels of Bcl-2 following etoposide treatment could not be inhibited by muscarinic receptor stimulation in CHO-M3 cells.
CHO-M3 cells seeded on to 10 cm2 plates were incubated with vehicle or etoposide (250 μM) for 16 h in the absence or presence of carbachol (1 mM). Total RNA was then extracted and reverse transcribed. RT-PCR using primers specific for Bcl-2 and the internal control β-actin was performed using SYBR® Green mastermix. Levels of the Bcl-2 transcript were then normalized to β-actin and expressed as a percentage of the control vehicle-treated cells. Results are means±S.E.M. (n=3).