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Total cell protein was separated by SDS/PAGE and immunoblotted with polyclonal anti-UDP-GlcDH as described in the Experimental section. –, E. coli expressing plasmid alone; +, E. coli expressing UDP-GlcDH; C, C. neoformans; T, T. mesenterica; S, S. cerevisiae. Standards (in kDa) are shown on the left. Regions of the gel not shown displayed no specific bands corresponding to UDP-GlcDH.
