Table 1. Primers used to generate mutations to alanine at the indicated positions.

All sequences are listed 5′ to 3′.

Mutant	5′-primer	3′-primer
C278A	GGCGTCTGTCGGTTTCGGTGGTAGCGCTTTCCAGAAGGACATTCTCAACTTGG	CCAAGTTGAGAATGTCCTTCTGGAAAGCGCTACCACCGAAACCGACAGACCC
R43A	GACCTCAACCAGCAGGCAATTCACGCCTGGAAC	GTTCCAGGCGTCAATTGCCTGCTGGTTGAGGTC
R262A	GTCGGTAAGGACACGGCAATGGGTTCCAAGTTCC	GGAACTTGGAACCCATTGCCGTGTCCTTACCGAC
K341A	CTTGGTTGGGCCTTCGCGAAGGACACTGGTGAC	GTCACCAGTGTCCTTCGCGAAGGCCCAACCAAG
R348A	GGACACTGGTGACACTGCAGAGTCTCCTTCCATCGGC	GCCGATGGAAGGAGACTCTGCAGTGTCACCAGTGTCC