Table 2. Inhibitors of UDP-GlcDH.
Assays were performed in the presence of the indicated compounds, as described in the Experimental section using 1 mM UDP-glucose. The amount of UDP-GlcA formed is expressed as a percentage of an assay with no additions. Results are expressed as means±S.D. for triplicate samples and are representative for at least four independent experiments.
| Addition | Concentration (mM) | % of control |
|---|---|---|
| 0 time control | – | 0 |
| – | – | 100±4 |
| NADH* | 1 | 1.3±0.2 |
| UDP-Xyl | 1 | 0.5±0.1 |
| UDP-GlcA† | 1 | 75±5 |
| UDP | 1 | 111±2 |
| UDP-Gal | 1 | 98±6 |
| Mn2+ | 1 | 95±5 |
| Mn2+ | 10 | 19±2 |
| EDTA | 1 | 110±4 |
* Lactate dehydrogenase and pyruvate were omitted from this assay.
† The product peak in this reaction was corrected for the amount of UDP-GlcA added.