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. 2004 Jun 22;381(Pt 1):213–219. doi: 10.1042/BJ20031506

Figure 7. CLAN inhibits caspase 1 activation induced by Nod2.

Figure 7

(A) HEK-293T cells were transfected with plasmids encoding pro-IL-1β (400 ng) and caspase 1 (100 ng), with (+) or without (−) Nod2 (50 ng) or CLAN [+, 50 ng; ++, 500 ng]. Supernatants were analysed for IL-1β secretion by ELISA at 24 h post-transfection. *P<0.05, relative to Nod2 alone. (B) HEK-293T cells were transfected with plasmids encoding pro-IL-1β (400 ng) and caspase 1 (100 ng), with (+) or without (−) Nod2 (50 ng), CLAN (400 ng), CLAN[NACHT] (400 ng), Apaf-1[NB-ARC] (400 ng) or caspase 4 (100 ng), as indicated. Supernatants were analysed for IL-1β secretion by ELISA at 24 h post-transfection. (C) HEK-293T cells were transfected with plasmids encoding pro-IL-1β (400 ng) and caspase 1 (100 ng), with (+) or without (−) CLAN[ΔLRR] (100 ng [+] or 500 ng [++]), Nod2[ΔLRR] (100 ng), CLAN[NACHT] (500 ng) or ASC (200 ng). Supernatants were analysed for IL-1β secretion by ELISA 24 h post-transfection. *, Experimental conditions that resulted in significantly less IL-1β secretion compared with Nod2 alone (P<0.05). Results are means±S.D. representative of at least two independent experiments.