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. 2004 Jun 22;381(Pt 1):213–219. doi: 10.1042/BJ20031506

Figure 8. CLAN and Nod2 co-associate in monocytes following exposure to PGN.

Figure 8

THP-1 cells expressing epitope-tagged CLAN (THP-1/CLAN) or neomycin-resistant control cells (THP-1/Neo) were exposed to PGN (10 μg/ml) for the times indicated, then lysed, and subjected to anti-myc immunoprecipitation and immunoblotting for detection of associated Nod2 protein. To assess equivalent immunoprecipitation of CLAN, membranes were stripped and re-probed with anti-myc antibodies. As a control, some samples were subjected to immunoprecipitation with normal mouse IgG (lanes 1 and 4). The blot is representative of three independent experiments.