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. 2004 Jun 22;381(Pt 1):321–328. doi: 10.1042/BJ20040108

Figure 4. Acetylation activity towards ISP-1 of Sli1p-overexpressing cell lysate.

Figure 4

(A) Chemical structure of ISP-1. (B) ISP-1 acetyltransferase activity of Sli1p-overexpressing cells. Cell lysates prepared from sli1-null cells containing YEp351 (Vector) or YEp351ADH1-Sli1 (SLI1) were used as enzyme sources (0.5 μg/μl). Sli1p was overexpressed under the ADH1 promoter. Samples were incubated for 5 min at 30 °C with (left and middle lanes) or without (right lane) ISP-1. Products were extracted and separated on a TLC plate as described in the Experimental section using solvent A. A major putative band of acetylated ISP-1 is indicated by an arrowhead. Authentic standards are also indicated. (C) The radioactive acetyl-ISP-1 was treated under alkaline conditions as described in the Experimental section and separated on a TLC plate using solvent B. A major putative band of acetylated ISP-1 is indicated by an arrowhead. Authentic standards are also indicated.