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. 2004 Aug 24;382(Pt 2):425–431. doi: 10.1042/BJ20040948

Figure 6. Association between PP2Cδ and members of the RSK and MSK family of kinases.

Figure 6

COS-7 cells were co-transfected with plasmids expressing myc–tagged PP2Cδ (H154D,T333A) and HA-tagged protein kinases. Transfection of myc–PP2Cδ(H154D,T333A) without HA-tagged protein kinase was used as a control. After 48 h, the cells were lysed and the protein kinases were precipitated using anti-HA antibody. The samples were washed five times and subjected to SDS/PAGE followed by immunoblotting with anti-myc or anti-HA antibody. The expression level of PP2Cδ in the cells was determined by immunoblotting of lysates with anti-myc antibody.