Figure 1. Fold-purification of plasma membranes and caveolae/raft domains.

Left-hand panel: plasma membranes were isolated and the purity was compared with that of cell homogenates by analysis of Western blots with anti-Na⁺,K⁺-ATPase as described in the Materials and methods section. Right-hand panel: caveolae/raft domains were isolated from the plasma membranes without the use of detergents as described in the Materials and methods section. The purity of caveolae/rafts was compared with that of plasma membranes by analysis of Western blots with anti-caveolin-1 or measurement of DHE/mg of protein as described in the Materials and methods section.