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. 2004 Aug 24;382(Pt 2):451–461. doi: 10.1042/BJ20031562

Figure 2. Sterol exchange between membranes.

Figure 2

(A) Effects of SCP-2 on sterol transfer from plasma membranes. DHE exchange between plasma membrane donors and plasma membrane acceptors was measured by monitoring polarization as described in the Materials and methods section. Open circles show the spontaneous sterol transfer from donor plasma membranes after the addition of a 10-fold excess of acceptor plasma membranes. Closed circles show the effects of 1.5 μM SCP-2 on the sterol transfer from donor plasma membranes to a 10-fold excess of acceptor plasma membranes. (B) Effects of SCP-2 on sterol transfer from caveolae/raft domains. DHE exchange was measured as in (A), except that caveolar/raft domain donor and caveolar/raft domain acceptor membranes were used as described in the Materials and methods section. Open circles show the spontaneous sterol transfer from donor caveolae after addition of a 10-fold excess of acceptor caveolae/raft domains. Closed circles show the effect of 1.5 μM SCP-2 on the sterol transfer from donor caveolae/rafts to a 10-fold excess of acceptor caveolae/rafts.