Table 3. Transbilayer structure of caveolae/raft domains and plasma membranes: DPH probes (DPH, TMA-DPH and DPH-Pro).
DPH, TMA-DPH, and DPH-Pro were incorporated at low, non-self-quenching levels into purified plasma membranes and caveolae/rafts as described in the Materials and methods section. Relative fluorescence intensity and polarization values are means±S.D. (n=7).
| Membrane | Relative fluorescence intensity | Polarization |
|---|---|---|
| DPH | ||
| Caveolae/rafts | 95.2±2.7* | 0.3085±0.0008*† |
| Plasma membranes | 87.7±1.9 | 0.2689±0.0009 |
| DPH-TMA | ||
| Caveolae/rafts | 98.5±2.8* | 0.2577±0.0.0007*‡ |
| Plasma membranes | 87.9±1.8 | 0.2658±0.0025 |
| DPH-Pro | ||
| Caveolae/rafts | 100.0±1.9* | 0.2896±0.0032* |
| Plasma membranes | 88.2±2.5 | 0.2731±0.0011 |
* P<0.01 compared with plasma membrane.
† P<0.01 compared with DPH-TMA.
‡ P<0.01 compared with DPH-Pro.