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. 2004 Aug 24;382(Pt 2):463–470. doi: 10.1042/BJ20040471

Figure 3. Effects of variable spacer lengths between the C/EBP- and FoxA-binding sites in the GRU.

Figure 3

A series of GRU constructs was made with deletions or insertions between the C/EBP and FoxA REs. These modified GRUs were placed upstream of the proximal enhancer and minimal promoter of the CPS gene, and tested by transient transfection into FTO-2B hepatoma cells. After 24 h of treatment with or without glucocorticoids, cells were lysed and luciferase values were measured. The results are expressed as means±S.E.M. and fold induction from at least four experiments. Results that are significantly different from those with the parent construct c are indicated by asterisks. Shortening of the C/EBP–FoxA spacer (constructs a and b) significantly increased activity relative to the parent construct (c), whereas increasing the distance had no effect (constructs d–f). Dex, dexamethasone.