Figure 3. Activation of NFκB by TNFα and insulin.

Double-stranded labelled oligonucleotides with the sequence of a consensus NFκB-binding site (NFcons) were incubated with 8 μg of nuclear extract protein from H4IIE cells treated with TNFα (A, B) or insulin (B) for the indicated times. Supershift experiments were carried out by preincubation of the nuclear extracts with 100 ng of anti-p65/RelA antibody. The arrow indicates the NFκB-containing complex.