Figure 7. Identification of NFκB-binding sites within the G6Pase promoter.

(A) Comparison of the inverted consensus sequence of a NFκB-binding site with the G6Pase promoter sequences −1193/−1184 (NF1) and −155/−146 (NF2). (B, C). EMSAs using double-stranded oligonucleotides with the sequence of (B) NF1 and (C) NF2, NF2mut or NFcons as probes. Nuclear extracts were isolated from H4IIE cells treated with or without TNFα, as indicated. Supershift experiments were carried out by preincubation of the nuclear extracts with 100 ng of anti-p65/RelA or anti-p50 antibodies. In competition experiments, a 100-fold molar excess of the unlabelled double-stranded probe was used. Arrows indicate NFκB-containing complexes.