Figure 8. Effects of different mutations of the G6Pase promoter on regulation by TNFα.
H4IIE cells were co-transfected with 8.5 μg/dish of the indicated reporter gene plasmids and 0.5 μg/dish of pRL control plasmid in the presence or absence of TNFα (10 ng/ml) and the presence of Bt2cAMP (500 μM) and dexamethasone (1 μM). Data are presented as means±S.E.M. (n=3) of promoter activity in the presence of TNFα compared with the luciferase activity of the respective construct in the absence of TNFα, which was set as 100. All constructs show a significant inhibition by TNFα.