Skip to main content
. 2004 Aug 24;382(Pt 2):471–479. doi: 10.1042/BJ20040160

Figure 9. Role of DNA binding and protein–protein interactions on regulation of G6Pase promoter activity by NFκB.

Figure 9

(A) Western Blot analysis of HEK293 cells transiently transfected with 10 μg of empty vector (lane 1), wild-type pRSV-NFκB (lane 2), pRSV-NFκB-S276A (lane 3) or pRSV-NFκB-Y36F (lane 4). Western blotting was performed using an antibody against the p65/RelA subunit of NFκB. (B, C) H4IIE cells were co-transfected with 8.5 μg/dish of the reporter gene plasmids G6Pase(−1227/+57) (B) or pGL-TK-2×NFκB (C) together with 0.5 μg/dish of pRL control plasmid and 2 μg/dish of pRSV-NFκB, pRSV-NFκB-Y36F, pRSV-NFκB-S276A or pRSV-βgal in the presence or absence of TNFα as indicated. Data are presented as means±S.E.M. (n=3) of promoter activity compared with the respective luciferase activity in cells transfected with pRSV-βgal without TNFα, which was set as 100; *P<0.05 compared with expression in the presence of pRSV-βgal and in the absence of TNFα; **P<0.05 compared with expression in the presence of pRSV-βgal and pRSV-NFκB.

HHS Vulnerability Disclosure