Figure 2. Effects of phosphate, FCCP and malonate on the components of Δp and on the H₂O₂ production rate.

Incubation conditions were the same as those described in the legend to Figure 1(a). Open symbols (a,c,e) represent potentials (squares, Δp; triangles, Δψ; diamonds, ΔpH). Closed symbols (b,d,f) represent H₂O₂ production rate (squares, 100 nM nigericin present; diamonds, nigericin absent). Data points represent means±S.E.M. for measurements on four to six separate mitochondrial preparations. Phosphate (P_i) was added as KH₂PO₄ and malonate was added as the K⁺ salt. *Significant (P<0.05) difference from the datum point at zero concentration of P_i/FCCP/malonate for each respective panel.