Table 1. Specific marker enzyme activities in subcellular fractions from rat liver (n=3).
NADPH:cytochrome c reductase activity in nmol of cytochrome c reduced/mg of protein per min; glucose-6-phosphatase activity in μmol of Pi formed/mg of protein per min; monoamine oxidase activity in nmol of benzylamine oxidized/mg of protein per min; cytochrome c oxidase activity in nmol of cytochrome c oxidized/mg of protein per min. ND, not determined.
| Fraction | NADPH:cytochrome c reductase (microsomes) | Glucose-6-phosphatase (MAM) | Monoamine oxidase (outer membranes) | Cytochrome oxidase (inner membranes) |
|---|---|---|---|---|
| Microsomes | 35.2±8.2 | 139±33 | ND | ND |
| MAM | 14.1±2.8 | 298.5±76 | 0.69±0.12 | 17.3±2.5 |
| Whole mitochondria | 0.70±0.08 | 23.9±2.7 | 11.5±0.9 | 427±23 |
| Outer membranes | 1.05±0.10 | 15.2±0.9 | 36.8±2.7 | 27.1±3.3 |
| Inner membranes | 0.15±0.02 | 1.25±0.11 | 1.84±0.12 | 1750±160 |