Table 1.
The information on primers used for 7 MLST housekeeping genes.
| Housekeeping gene |
Primer |
||||
|---|---|---|---|---|---|
| Target1 | Abbreviation | Orientation | Sequence (5′–3′) | Length (nt) | Amplicon size (bp) |
| adenylate kinase | adk | Forward | ATTCTGCTTGGCGCTCCGGG | 20 | 584 |
| Reverse | CCGTCAACTTTCGCGTATTT | 20 | |||
| fumarate hydratase | fumC | Forward | ACAGGTCGCMAGCGCTTCAA | 20 | 737 |
| Reverse | CTCARCGCTCGCTGGAGCATT | 21 | |||
| DNA gyrase subunit B | gyrB | Forward | TCGGCGACACGGATGACGGC | 20 | 880 |
| Reverse | ATCAGGCCTTCACGCGCATC | 20 | |||
| isocitrate dehydrogenase | icd | Forward | ATGGAAAGTAAAGTTGTTCCGGCACA | 26 | 878 |
| Reverse | GGACGCAGCAGGATCTGTT | 19 | |||
| malate dehydrogenase | mdh | Forward | ATGAAAGTCGCAGGCGCTGCTGGCGG | 26 | 932 |
| Reverse | TTAACGAACTCCTCGATATCTTTCTT | 26 | |||
| adenylosuccinate dehydrogenase | purA | Forward | GCCGCGCTGATGAAAGAGATG | 21 | 820 |
| Reverse | GCATACGGTAAGCCACRCAGA | 21 | |||
| DNA recombination/repair protein | recA | Forward | CGCATTCGCTTTACCYTGACC | 21 | 734 |
| Reverse | TCGTCGAAATCTACGGACCRGA | 22 | |||
1
The reference sequence was based on Escherichia coli str. K-12 substr. MG1655, complete genome (Accession No.: NC_000913.3) and avian pathogenetic E. coli whole-genome sequencing results conducted for this study.