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. 2004 Aug 24;382(Pt 2):677–685. doi: 10.1042/BJ20040358

Figure 3. Binding of increasing concentrations of purified, fluorescently labelled full-length ankyrin-binding domain and its mutants by PE/PC (3:2) FAT-liposomes.

Figure 3

Curves were fitted by non-linear regression according to the equation B=Bmax×free/KD+free. B and Bmax denote protein bound; the concentration of free protein is given in mol/l. Details in are given in the Materials and methods section.