Table 2. Equilibrium dissociation constants for binding of ankyrin by expressed full-length ankyrin-binding domain (DWA) and its truncated mutants (Frag.1, Frag.3 and Frag.5).

Data were obtained from hyperbolic binding isotherms (not shown) by non-linear regression according to the equation: B=B_max·free/K_D+free; B and B_max, bound ankyrin, the concentration of free ankyrin is in mol/l. Values are means±S.E.M.

Protein	KD (nM)
DWA	173±28
Frag.1	169±37
Frag.3	370±75
Frag.5	677±450