Table 3. Kinetic parameters for the interaction of the full-length ankyrin-binding domain and its mutants with PE/PC mono- and bi-layers obtained from SPR measurements (Figure 5) and pelleting assay (Figure 3).

The parameters were determined as described in the Materials and methods section. In addition, K_D and R_max values for the interaction of the full-length ankyrin-binding domain and Frag.5 with the hydrophobic surface of a bare chip (hexadecane thiol-covered) are presented.

	Pelleting assay (Figure 3)		SPR (PE/PC) (Figure 5)		Hydrophobic surface
Protein	KD (μM])	Bmax (μg/mg of phospholipid)	KD (μM)	Rmax	KD (μM)	Rmax
DWA	0.05	3.81	0.27	124	0.449	127
N1C	0.107	3.62	0.234	119
FRAG.1	0.25	5.57	0.363	124
FRAG.3	0.18	11.9	0.25	978
FRAG.5	0.79	38.8	0.18	516	27.8	6910