Figure 4. Specificity of ABCG2 photolabelling with IAARh123.
(A) Membrane proteins (50 μg) from MCF-7/AdrVp1000 cells were photolabelled with increasing concentrations (0–4 μM) of IAARh123. After SDS/PAGE, the bands corresponding to ABCG2 were excised and quantified. (B) Photolabelled ABCG2 from MCF-7/AdrVp1000 cells incubated in the absence or presence of a molar excess (0–1000-fold) of IAARh123. (C) Photolabelling of ABCG2 with IAARh123 in the absence or presence of reserpine at a 300× molar excess.