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. 2004 Aug 24;382(Pt 2):733–740. doi: 10.1042/BJ20031453

Figure 1. Comparison of gel mobility shift assay with unlabelled versus ANTS-labelled oligosaccharides.

Figure 1

dp12 oligosaccharides were either unlabelled (lanes 1, 2 and 3) or derivatized with ANTS (lanes 4, 5 and 6) and next subjected to electrophoresis in the absence or presence of CyPB. Lane 1, 10 nmol of unlabelled oligosaccharides; lane 2, 5 nmol of unlabelled oligosaccharides plus 1 nmol of CyPB; lane 3, 10 nmol of unlabelled oligosaccharides plus 1 nmol of CyPB; lane 4, 10 nmol of ANTS-labelled oligosaccharides; lane 5, 5 nmol of ANTS-labelled oligosaccharides plus 1 nmol of CyPB; lane 6, 10 nmol of ANTS-labelled oligosaccharides plus 1 nmol of CyPB. (A) The electrophoretic profile was revealed by staining with aqueous Azure A. (B) Fluorophore-assisted detection of the migration of ANTS-labelled dp12 oligosaccharides (exposure to a UV transilluminator, 0.24 s). A representative gel of three separate experiments is shown.