Figure 3. Viscosity effect on kinetic parameters of wild-type and mutant enzymes.

The effect of viscosity on kinetic parameters was assayed by using 0.1 M potassium phosphate buffer, pH 6.5, with various glycerol concentrations. Dependence of the reciprocal of the relative catalytic-centre activity (k_cat^o/k_cat) on the relative viscosity (η/η^o) for CDNB as co-substrate. D3, wild-type.