Table 2. Effect of fluoride/chloride leaving group substitution on the rate of catalysis.
Enzyme | kcatFDNB (s−1) | kcatCDNB (s−1) |
---|---|---|
Wild-type | 85.1 | 35.4 |
I52A | 27.5 | 16.9 |
S65A | 157.3 | 29.8 |
R66A | 18.1 | 3.3 |
M101A | 265.9 | 63.0 |
Enzyme | kcatFDNB (s−1) | kcatCDNB (s−1) |
---|---|---|
Wild-type | 85.1 | 35.4 |
I52A | 27.5 | 16.9 |
S65A | 157.3 | 29.8 |
R66A | 18.1 | 3.3 |
M101A | 265.9 | 63.0 |