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The catalytic-centre activities of the conjugation reaction catalyzing by adGST D3-3 enzymes of GSH with CDNB and FDNB as co-substrates were calculated at pH 6.5.
| Enzyme | kcatFDNB (s−1) | kcatCDNB (s−1) |
|---|---|---|
| Wild-type | 85.1 | 35.4 |
| I52A | 27.5 | 16.9 |
| S65A | 157.3 | 29.8 |
| R66A | 18.1 | 3.3 |
| M101A | 265.9 | 63.0 |
